One step antibody-mediated isolation and patterning of multiple cell types in microfluidic devices     

Danny Bavli  Elishai Ezra  Daniel Kitsberg  Margarita Vosk-Artzi  Shashi K. Murthy  Yaakov Nahmias 《Biomicrofluidics》2016,10(2)

Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.  相似文献   

From the President     

Kay A. Persichitte 《TechTrends》2016,60(1):4-4

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CURRENT AND FUTURE SCHOOL PSYCHOLOGISTS’ PREPAREDNESS TO WORK WITH LGBT STUDENTS: ROLE OF EDUCATION AND GAY‐STRAIGHT ALLIANCES     

Prerna G. Arora  Jennifer Kelly  Thalia R. Goldstein 《Psychology in the schools》2016,53(7):722-735

This study sought to assess current and future school psychologists’ attitudes toward and preparedness to address the needs of lesbian, gay, bisexual, and transgender (LGBT) students in schools. Two‐hundred seventy‐nine school psychologists (n = 162, 58%) and school psychology graduate students (n = 117, 42%) were included in the study. Participants completed measures of attitudes toward LGBT students, preparedness to address the needs of LGBT youth, and social desirability. For both school psychologists and graduate students, increased education was associated with improved attitudes and increased preparedness to treat LGBT youth. For school psychologists, presence of a gay‐straight alliance was associated with increased knowledge about LGBT youth, as well as higher ratings of preparedness to treat LGBT youth; the same associations did not hold true for the graduate students. These findings have implications for the training and practice of school psychologists in addressing the needs of LGBT youth in schools.  相似文献   

Understanding the programmatic and contextual forces that influence participation in a government-sponsored international student-mobility program     

Laura W. Perna  Kata Orosz  Zakir Jumakulov  Marina Kishkentayeva  Adil Ashirbekov 《Higher Education》2015,69(2):173-188

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Father of Route 66: The Story of Cy Avery Susan Croce Kelly. Norman: University of Oklahoma Press, 2014.          下载免费PDF全文

Daniel P. Murphy 《Journal of American culture (Malden, Mass. : 2003)》2016,39(4):460-461

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Test Development with Performance Standards and Achievement Growth in Mind     

Steve Ferrara  Dubravka Svetina  Sylvia Skucha  Anne H. Davidson 《Educational Measurement》2011,30(4):3-15

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Efficacy of the TELL language and literacy curriculum for preschoolers with developmental speech and/or language impairment     

M. Jeanne Wilcox  Shelley I. Gray  Amy B. Guimond  Addie E. Lafferty 《Early childhood research quarterly》2011,26(3):278-294

The purpose of this investigation was to examine the efficacy of a new preschool oral language and early literacy curriculum package (Teaching Early Literacy and Language [TELL]) for children with developmental speech and/or language impairment (DSLI) either as a primary (e.g., specific to speech and/or language) or secondary impairment (e.g., developmental delay that includes DSLI). Participants included 118 children (30 females, 88 males, M age = 53.58 months) with DLSI and their 29 preschool teachers. The design was a randomized controlled trial (RCT) with assignment to experimental versus contrast conditions at the classroom level. Teachers in TELL classes received formal training, in-class support, and mentoring to implement the curriculum. Dependent measures for the children included scores on the Clinical Evaluation of Language Fundamentals-Preschool 2nd edition (CELF-P2), the Phonological Awareness Literacy Screening for Preschool (PALS-PreK), the Renfrew Bus Story (BUS), and a receptive and expressive vocabulary measure developed for this investigation (VOCAB). Results indicated that when compared to the contrast group, children in the TELL condition demonstrated greater gains on the phonological awareness subtest of the CELF-P2, the sentence length score of the BUS, the letter sounds, beginning sound awareness, and rhyme awareness subtests of the PALS-PreK, and VOCAB. Results suggest that the TELL curriculum package has promise for promoting gains in early literacy and oral language skills in preschool children with DLSI.  相似文献   

Educational Programs for Elementary Students with Learning Disabilities: Can They Be Both Effective and Inclusive?     

James McLeskey  Nancy L. Waldron 《Learning disabilities research & practice》2011,26(1):48-57

One of the most controversial issues in special education over the last 40 years has been the extent to which students with learning disabilities (LD) should be educated in general education classrooms. Recent mandates in federal law requiring that all students with disabilities have access to the general education curriculum and make adequate yearly progress relative to this content have intensified this debate. In this article, a brief summary of research regarding the nature of instruction that produces significantly improved educational outcomes for students with LD is provided. This is followed by a review of research related to the delivery of this high‐quality instruction in inclusive, general education classrooms and in resource settings. We conclude that this research provides the foundation for reconsidering full inclusion and how services are delivered for elementary students with LD.  相似文献   

Political Incongruity between Students’ Ideological Identity and Stance on Specific Public Policies in a Predominantly White Southeastern State Institution     

Jeremy T. Coles  Brittany A. Carstens  Jennifer M. Wright  Robert L. Williams 《Innovative Higher Education》2015,40(1):5-18

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Natural kinds no longer are what they never were     

Thomas A. C. Reydon 《Metascience》2015,24(2):259-264

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